Device

Part:BBa_K1368015

Designed by: xiaoxue guo   Group: iGEM14_HZAU-China   (2014-09-30)

Cre recombinase with post-transcriptional control driven by IPTG

We built this device as a input module. To reduce the leakage of the pLlac0-1 promoter, we added the crRNA and taRNA in it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61


[edit]
Categories
Parameters
n/aCre recombinase with post-transcriptional control driven by IPTG